eng URI http://hdl.handle.net/10795/1571 υλικά προηγμένης τεχνολογίας νανοτεχνολογία Insulin (INS) was encapsulated into complexes with poly(ethylene glycol)-block poly(L-lysine) (PEG-b-PLys), which is a polypeptide-based block copolymer (a neutral-cationic block polyelectrolyte). These macromolecules can encapsulate INS molecules in aqueous conditions via electrostatic interactions. Light scattering techniques are used in order to examine the complexation process of the hybrid nanoparticles in a gamut of buffers, as a function of protein concnetration. The physicochemical and structural characteristics of the complexes depend on the ionic strength of the aqueous medium, while the concentration of PEG-b-PLys was constant through the series of solutions. As INS concentration increased each polyelectrolyte chain interacts with an increasing number of INS molecules, the degree of charge neutralization becomes higher and the size distribution of the complexes decreased also, especially at the highest ionic strength. The size/structure of complexes diluted in biological medium indicated that the copolymer imparts stealth properties and colloidal and biological stability to the complexes, which could in turn affect the clearance properties in vivo. Therefore, these studies could be a rational roadmap for designing the optimum complexes/effective nanocarriers for proteins and peptides. 28 pp. LOMv1.0 creator LOMv1.0subject matter expert LOMv1.0 Project Final Beneficiary LOMv1.0 Project Executing Organisation 2015 Digital Library of the Operational Programme "Education and Lifelong Learning" abstract types Text Insulin Nanocarriers Poly(L-lysine) Derivatives Stability Micelles Lysozyme 832237 application/pdf http://repository.edulll.gr/edulll/bitstream/10795/1571/2/1571_J%20Phys%20Chem%20B%202015%206813.pdf URI http://hdl.handle.net/10795/1571 LOMv1.0creator 2016-01-22T10:07:53Z LOMv1.0validator 2016-01-22T10:07:53Z LOMv1.0 gre no no Copyright EYD-EPEDBM (Operational Programme "Education and Lifelong Learning")