eng URI http://hdl.handle.net/10795/1593 υλικά προηγμένης τεχνολογίας νανοτεχνολογία We present a study of the interaction of the positively charged model protein lysozyme with the negatively charged amphiphilic diblock polyelectrolyte micelles of poly(tert-butylstyrene)-bsodium (sulfamate/carboxylate)isoprene) (PtBS-b-SCPI) on the surface of silver. The adsorption kinetics are monitored by surface plasmon resonance and the surface morphology by atomic force microscopy. The micellar adsorption kinetics is dictated by two processes and the micellar layer morphology shows that the micelles do not lose their integrity upon adsorption. The complexation of lysozyme with the adsorbed micellar layers depends on the micelles arrangement and density in the underlying layer and lysozyme follows the local morphology of the underlying roughness. When the micellar adsorbed amount is small, the layers show low capacity in protein binding and low resistance in loading. When the micellar adsorbed amount is high the situation is inversed. The adsorbed layers both with or without added protein are found to be irreversibly adsorbed on the Ag surface. 32 pp. LOMv1.0 creator LOMv1.0subject matter expert LOMv1.0 Project Final Beneficiary LOMv1.0 Project Executing Organisation 2015 Digital Library of the Operational Programme "Education and Lifelong Learning" abstract types Text Plasmon resonance Adsorption kinetics Atomic force microscopy Micelles Lysozyme Proteins Silver 1026670 application/pdf http://repository.edulll.gr/edulll/bitstream/10795/1593/2/1593_Langmuir%202015%20685.pdf URI http://hdl.handle.net/10795/1593 LOMv1.0creator 2016-01-25T11:11:28Z LOMv1.0validator 2016-01-25T11:11:28Z LOMv1.0 gre no no Copyright EYD-EPEDBM (Operational Programme "Education and Lifelong Learning")