eng URI http://hdl.handle.net/10795/2315 biodegradability waste management This work investigated biostimulation and bioaugmentation as strategies for removing polyurethane (PU) waste in soil. Soil microcosms were biostimulated with the PU dispersion agent “Impranil” and/or yeast extract or were bioaugmented with PU-degrading fungi, and the degradation of subsequently buried PU was determined. Fungal communities in the soil and colonizing buried PU were enumerated on solid media and were analyzed using denaturing gradient gel electrophoresis (DGGE). Biostimulation with yeast extract alone or in conjunction with Impranil increased PU degradation 62% compared to the degradation in untreated control soil and was associated with a 45% increase in putative PU degraders colonizing PU. Specific fungi were enriched in soil following biostimulation; however, few of these fungi colonized the surface of buried PU. Fungi used for soil bioaugmentation were cultivated on the surface of sterile wheat to form a mycelium-rich inoculum. Wheat, when added alone to soil, increased PU degradation by 28%, suggesting that wheat biomass had a biostimulating effect. Addition of wheat colonized with Nectria haematococca, Penicillium viridicatum, Penicillium ochrochloron, or an unidentified Mucormycotina sp. increased PU degradation a further 30 to 70%, suggesting that biostimulation and bioaugmentation were operating in concert to enhance PU degradation. Interestingly, few of the inoculated fungi could be detected by DGGE in the soil or on the surface of the PU 4 weeks after inoculation. Bioaugmentation did, however, increase the numbers of indigenous PU-degrading fungi and caused an inoculum-dependent change in the composition of the native fungal populations, which may explain the increased degradation observed. These results demonstrate that both biostimulation and bioaugmentation may be viable tools for the remediation of environments contaminated with polyurethane waste. 11 pp. LOMv1.0 creator LOMv1.0 Project Final Beneficiary LOMv1.0 Project Executing Organisation 2010-02 LOMv1.0 training LOMv1.0 higher education 18+ Digital Library of the Operational Programme "Education and Lifelong Learning" abstract types Text biostimulation bioaugmentation polyurethane yeast extract fungi 1270117 application/pdf http://repository.edulll.gr/edulll/bitstream/10795/2315/2/2315_Biostimulation%20vs%20Bioaugmentation.pdf Πανεπιστήμιο Θεσσαλίας. Σχολή Επιστημών Υγείας. Τμήμα Βιοχημείας και Βιοτεχνολογίας. Βιβλιογραφία διαλέξεων. Πρόγραμμα δια βίου μάθησης ΑΕΙ για την επικαιροποίηση γνώσεων αποφοίτων ΑΕΙ (ΠΕΓΑ) «Οι σύγχρονες τεχνικές βιο-ανάλυσης στην υγεία, τη γεωργία, το περιβάλλον και τη διατροφή» - Ενότητα 2. Σύγχρονες τεχνικές βιοανάλυσης στη γεωργία και το περιβάλλον URI http://hdl.handle.net/10795/2315 LOMv1.0creator 2016-03-15T08:17:14Z LOMv1.0validator 2016-03-15T08:17:14Z LOMv1.0 gre no no Copyright EYD-EPEDBM (Operational Programme "Education and Lifelong Learning")