eng URI http://hdl.handle.net/10795/2627 βιοτεχνολογία βιοχημεία ένζυμο We developed a combinatorial strategy aiming at designing peptide inhibitors against the hGSTP1-1 isoenzyme involved in MDR. We developed a combinatorial strategy aiming at designing peptide inhibitors against the hGSTP1-1 isoenzyme. We employed a combinatorial peptide library featuring engineered E. coli cells harboring a plasmid able to express a fusion protein containing random 12peptides. After five selection rounds, clones were screened for hGSTP1-1 binding (dot blot hybridization) and those with the strongest signal were selected and sequenced. Sequence alignments showed a core binding sequence which, along with selected peptide fragments, were synthesized using the solid phase methodology . The synthetic peptides were studied for their inhibition potency against three human GSTP1-1 allozymes, A, B and C 1 pp. LOMv1.0 creator LOMv1.0 Project Final Beneficiary LOMv1.0 Project Executing Organisation 2015 Digital Library of the Operational Programme "Education and Lifelong Learning" abstract types Text Peptide Synthetic peptides Inhibitors Human glutathione transferase 2371400 application/pdf http://repository.edulll.gr/edulll/bitstream/10795/2627/2/2627_Poster%2012.pdf URI http://hdl.handle.net/10795/2627 LOMv1.0creator 2016-04-13T13:02:00Z LOMv1.0validator 2016-04-13T13:02:00Z LOMv1.0 gre no no Copyright EYD-EPEDBM (Operational Programme "Education and Lifelong Learning")