eng URI http://hdl.handle.net/10795/2676 βιοτεχνολογία ένζυμο γενετική μηχανική Enzymes are biocatalysts evolved in nature to achieve the speed and coordination of nearly all the chemical reactions that define cellular metabolism necessary to develop and maintain life. The application of biocatalysis is growing rapidly, since enzymes offer potential for many exciting applications in industry. The advent of whole genome sequencing projects enabled new approaches for biocatalyst development, based on specialised methods for enzyme heterologous expression and engineering. The engineering of enzymes with altered activity, specificity and stability, using sitedirected mutagenesis and directed evolution techniques are now well established. Over the last decade, enzyme immobilisation has become important in industry. New methods and techniques for enzyme immobilisation allow for the reuse of the catalysts and the development of efficient biotechnological processes. This chapter reviews advances in enzyme technology as well as in the techniques and strategies used for enzyme production, engineering and immobilisation and discuss their advantages and disadvantages. 42 pp. LOMv1.0 creator LOMv1.0editor LOMv1.0 publisher LOMv1.0 Project Final Beneficiary LOMv1.0 Project Executing Organisation 2012-06 Digital Library of the Operational Programme "Education and Lifelong Learning" abstract types Text Enzymes Biocatalysis Enzyme engineering Enzyme technology 2826550 application/pdf http://repository.edulll.gr/edulll/bitstream/10795/2676/3/2676_PAPER4.pdf 2nd Edition URI http://hdl.handle.net/10795/2676 LOMv1.0creator 2016-04-15T09:13:59Z LOMv1.0validator 2016-04-15T09:13:59Z LOMv1.0 gre no no Copyright EYD-EPEDBM (Operational Programme "Education and Lifelong Learning")