eng URI http://hdl.handle.net/10795/2952 βιολογία γενετική μικροοργανισμός κυτταρολογία The nuclear ribosomal intergenic spacer (IGS) region was structurally analyzed and exploited for molecular discrimination and phylogenetic analysis of vegetative compatibility groups (VCGs) of Verticillium dahliae. A structural study of 201 available IGS sequences of the fungus was performed, and four classes of ubiquitous repetitive elements, organized in higher-order repetitive structures or composite blocks, were detected in a variable IGS subregion. This subregion was amplified from an international collection of 59 V. dahliae isolates covering all VCGs, together with nine representative V. albo-atrum and V. longisporum isolates, and sequenced. Structural and phylogenetic analyses of the sequences of this polymorphic IGS subregion were consistently informative and allowed the identification of two main lineages in V. dahliae, that is, clade I including VCGs 1A, 1B, 2A, 4B, and 3 and clade II containing VCGs 2B, 4A, and 6. Analysis of IGS sequences proved a highly suitable molecular tool for (a) rapid interspecific differentiation, (b) intraspecific discrimination among VCGs of V. dahliae, facilitating high-throughput VCG confirmation and prediction/profiling, and (c) phylogenetic analysis within and among V. dahliae VCGs. 10 pp. LOMv1.0 creator LOMv1.0 publisher LOMv1.0 Scientific Coordinator LOMv1.0 Project Executing Organisation 2013-07-29 Digital Library of the Operational Programme "Education and Lifelong Learning" abstract types Text IGS sequences molecular differentiation tools VCG discrimination VCG phylogenetic relationships http://onlinelibrary.wiley.com/doi/10.1111/1574-6968.12215/abstract 352038 application/pdf http://repository.edulll.gr/edulll/bitstream/10795/2952/2/2952_1.96_%ce%94%ce%97%ce%9c_29_7_13.pdf URI http://hdl.handle.net/10795/2952 LOMv1.0creator 2016-04-27T07:59:58Z LOMv1.0validator 2016-04-27T07:59:58Z LOMv1.0 gre no no Copyright EYD-EPEDBM (Operational Programme "Education and Lifelong Learning")