eng URI http://hdl.handle.net/10795/2592 ένζυμο βιοτεχνολογία ψυχανθές Three Glutathione transferase (GSTs) isoenzymes have been isolated from P. vulgaris leaves after in vivo treatment with 1/250 fluazifop-p-butyl herbicide. The inducible GST isoenzymes were identified and separated by affinity chromatography. They found to belong to phi and tau classes. Moreover, the fluazifop-inducible glutathione transferases from P. vulgaris (PvGSTs, termed PvGSTU2-2, PvGSTF1-1 and PvGSTU3-3) were found to catalyze a broad range of reactions and exhibit quite varied substrate specificity. Another GST isoenzyme was isolated from P. vulgaris (PvGST, termed PvGSTU3-3), after its induction with biotic stress treatment (Uromyces appendiculatus infection). PvGSTU3-3 shares high homology the tau class plant and catalyzes several different reactions and exhibits wide substrate specificity. Of particular importance are the high antioxidant catalytic function and hydroperoxidase, thioltransferase, and dehydroascorbate reductase action of Pv-GSTU3-3. Transgenic tobacco plants over-expressing PvGSTU2-2 isoenzyme have been developed via Agrobacteriun tumefaciens in order to study their in planta potential to confer biotic and abiotic resistance as a means of plant breeding. Our results provide new insights into catalytic and structural diversity of GSTs and the detoxifying mechanism used by P. vulgaris. Moreover, highlight the functional and catalytic diversity of plant GSTs and demonstrate their pivotal role for addressing biotic stresses in P. vulgaris. 1 pp. LOMv1.0 creator LOMv1.0 Project Final Beneficiary LOMv1.0 Project Executing Organisation 2015 Digital Library of the Operational Programme "Education and Lifelong Learning" abstract types Text GST isolation Glutathione Phaseolus vulgaris L. Abiotic stress Biotic stress Common Bean 747092 application/pdf http://repository.edulll.gr/edulll/bitstream/10795/2592/2/2592_Poster%202.pdf URI http://hdl.handle.net/10795/2592 LOMv1.0creator 2016-04-13T09:42:41Z LOMv1.0validator 2016-04-13T09:42:41Z LOMv1.0 gre no no Copyright EYD-EPEDBM (Operational Programme "Education and Lifelong Learning")